Protocadherin gamma antibody (AA 808-931) (Atto 488)

Catalog No. ABIN1741181

The Mouse Monoclonal anti-Protocadherin gamma antibody has been validated for IHC, WB, ICC and IF. It is suitable to detect Protocadherin gamma in samples from Mouse.

Quick Overview for Protocadherin gamma antibody (AA 808-931) (Atto 488) (ABIN1741181)

Target: Protocadherin gamma

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Protocadherin gamma antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S159-5

Product Details anti-Protocadherin gamma Antibody (Atto 488)

Binding Specificity: AA 808-931

Specificity: Detects ~100 kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids).

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1741181 was sufficient for detection of Protocadherin gamma (pan) in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Protocadherin gamma

Target: Protocadherin gamma

Background: The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Gene ID: 93709

NCBI Accession: NP_291062

UniProt: Q91XZ0