MFN2 antibody (AA 370-600) (PerCP)

Catalog No. ABIN1741248

This anti-MFN2 antibody is a Mouse Monoclonal antibody detecting MFN2 in WB, IHC, ICC and IF. Suitable for Human.

Quick Overview for MFN2 antibody (AA 370-600) (PerCP) (ABIN1741248)

Target: MFN2 (Mitofusin 2 (MFN2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MFN2 antibody is conjugated to PerCP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S153-5

Product Details anti-MFN2 Antibody (PerCP)

Binding Specificity: AA 370-600

Specificity: Detects ~90 kDa. No cross-reactivity against Mitofusin-1.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2. Rat: 97% identity (226/231 amino acids identical). Human: 92% identity (214/231 amino acids identical) ~55% identity with Mitofusin-1.

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1741248 was sufficient for detection of Mitofusin-2 in 20 μg of mouse cardiac mitochondrial lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for MFN2

Target: MFN2 (Mitofusin 2 (MFN2))

Alternative Name: Mitofusin 2

Background: Mitofusin-2 (Mfn2) is a membrane protein of the mitochondria and is important in mitochondrial fusion in mammalian cells. Mfn2 activity has also been implicated in mitochondrial metabolism and the loss of Mfn2 may be the reason for certain metabolic modifications that are seen in individuals with obesity. Mfn2 has been associated with maintaining the membrane potential of the mitochondria. Mfn2 has been shown to hinder mitochondrial antiviral signaling by inhibiting production of type 1 interferons. Mfn2 is a p53-inducible target and involved in cell proliferation, apoptosis promotion and tumor suppression.

Gene ID: 170731

NCBI Accession: NP_573464

UniProt: Q80U63

Pathways: Skeletal Muscle Fiber Development