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SHANK3 antibody (AA 538-626)

SHANK3 Reactivity: Rat WB, IHC, IF, ICC Host: Mouse Monoclonal S367-51 unconjugated
Catalog No. ABIN1741251
  • Target See all SHANK3 Antibodies
    SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))
    Binding Specificity
    • 15
    • 10
    • 9
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 538-626
    Reactivity
    • 43
    • 11
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 26
    • 21
    Mouse
    Clonality
    • 26
    • 21
    Monoclonal
    Conjugate
    • 13
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SHANK3 antibody is un-conjugated
    Application
    • 31
    • 27
    • 22
    • 21
    • 17
    • 13
    • 13
    • 10
    • 8
    • 5
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects ~190 kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3. Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2.
    Clone
    S367-51
    Isotype
    IgG2a
    Top Product
    Discover our top product SHANK3 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN1741251 was sufficient for detection of SHANK1/SHANK3 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Target
    SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))
    Alternative Name
    SHANK3 (SHANK3 Products)
    Synonyms
    DEL22q13.3 antibody, PROSAP2 antibody, PSAP2 antibody, SCZD15 antibody, SPANK-2 antibody, Prosap2 antibody, AI841104 antibody, Shank3b antibody, SH3 and multiple ankyrin repeat domains 3 antibody, SH3 and multiple ankyrin repeat domains protein 3 antibody, SHANK3 antibody, shank3 antibody, Shank3 antibody
    Background
    SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development.
    Gene ID
    59312
    UniProt
    Q9JLU4
    Pathways
    Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity
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