VAMP2 antibody (AA 2-17)

Catalog No. ABIN1742194

This Guinea Pig Polyclonal antibody specifically detects VAMP2 in WB, IHC, ICC and IP. It exhibits reactivity toward Human, Mouse, Rat and Hamster and has been mentioned in 3+ publications.

Quick Overview for VAMP2 antibody (AA 2-17) (ABIN1742194)

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Reactivity: Human, Mouse, Rat, Hamster

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This VAMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-VAMP2 Antibody

Binding Specificity: AA 2-17

Specificity: Specific for VAMP 2

Cross-Reactivity (Details): no cross reactivity to VAMP 1 and VAMP 3

Purification: antiserum

Immunogen: Synthetic peptide SATAATVPPAAPAGEG (aa 2-17 in rat synaptobrevin 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
ICC: 1 : 1000
IHC: 1 : 500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-VAMP2 antibody (ABIN1742194)

van Stegen, Dagar, Gottmann: "Release activity-dependent control of vesicle endocytosis by the synaptic adhesion molecule N-cadherin." in: Scientific reports, Vol. 7, pp. 40865, (2017) (PubMed).

Schaefer, Toral, Velez, Cox, Baker, Borcherding, Colgan, Bondada, Mashburn, Yu, Geddes, Tsang, Bassuk, Mahajan: "Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses." in: Investigative ophthalmology & visual science, Vol. 57, Issue 6, pp. 2509-21, (2016) (PubMed).

Kung, Gong, Adedoyin, Ng, Bhargava, Ohara, Jasmin: "Evidence for glutamate as a neuroglial transmitter within sensory ganglia." in: PLoS ONE, Vol. 8, Issue 7, pp. e68312, (2013) (PubMed).

Target Details for VAMP2

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Alternative Name: Synaptobrevin 2

Background: Synonyms: VAMP 2, p18-2

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport