RAB3A antibody (AA 154-227)

Catalog No. ABIN1742210

The Rabbit Polyclonal anti-RAB3A antibody has been validated for WB, IHC and ICC. It is suitable to detect RAB3A in samples from Human, Rat and Mouse. There are 3+ publications available.

Quick Overview for RAB3A antibody (AA 154-227) (ABIN1742210)

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-RAB3A Antibody

Binding Specificity: AA 154-227

Specificity: Specific for all four Rab 3 isoforms a, b, c, d.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant human Rab 3c (aa 154-227).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 1000
IHC: 1 : 200 up to 1 : 500

Comment:

This antibody has been affinity purified with the complete antigen (aa 154 - 227) and recognizes all isoforms, due to the homology of Rab 3 proteins.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-RAB3A antibody (ABIN1742210)

Romanov, Alpár, Zhang, Zeisel, Calas, Landry, Fuszard, Shirran, Schnell, Dobolyi, Oláh, Spence, Mulder, Martens, Palkovits, Uhlen, Sitte, Botting, Wagner, Linnarsson, Hökfelt, Harkany: "A secretagogin locus of the mammalian hypothalamus controls stress hormone release." in: The EMBO journal, Vol. 34, Issue 1, pp. 36-54, (2015) (PubMed).

Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." in: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, (2013) (PubMed).

de Wit, Walter, Milosevic, Gulyás-Kovács, Riedel, Sørensen, Verhage: "Synaptotagmin-1 docks secretory vesicles to syntaxin-1/SNAP-25 acceptor complexes." in: Cell, Vol. 138, Issue 5, pp. 935-46, (2009) (PubMed).

Target Details for RAB3A

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Alternative Name: Rab 3

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport