RAB3A antibody (AA 2-14)

Catalog No. ABIN1742211

This anti-RAB3A antibody is a Rabbit Polyclonal antibody detecting RAB3A in WB, ELISA, IHC, ICC and IP. Suitable for Human, Rat, Mouse, Zebrafish (Danio rerio), Cow and Hamster. This Primary Antibody has been cited in 4+ publications.

Quick Overview for RAB3A antibody (AA 2-14) (ABIN1742211)

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-RAB3A Antibody

Binding Specificity: AA 2-14

Specificity: Specific for Rab 3a.

Purification: antiserum

Immunogen: Synthetic peptide ASATDSRYGQKES (aa 2-14 in rat Rab 3a) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 1000

Comment:

ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-RAB3A antibody (ABIN1742211)

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Rastaldi, Armelloni, Berra, Li, Pesaresi, Poczewski, Langer, Kerjaschki, Henger, Blattner, Kretzler, Wanke, DAmico: "Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a." in: The American journal of pathology, Vol. 163, Issue 3, pp. 889-99, (2003) (PubMed).

Schlüter, Khvotchev, Jahn, Südhof: "Localization versus function of Rab3 proteins. Evidence for a common regulatory role in controlling fusion." in: The Journal of biological chemistry, Vol. 277, Issue 43, pp. 40919-29, (2002) (PubMed).

Von Kriegstein, Schmitz, Link, Südhof: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).

Target Details for RAB3A

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Alternative Name: Rab 3a

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport