RAB3A antibody (AA 2-14)

Catalog No. ABIN1742211

Cited in 4+ publications. The Rabbit Polyclonal anti-RAB3A antibody (ABIN1742211) specifically detects RAB3A in WB, ELISA, IHC, ICC and IP. The antibody is reactive with Human, Rat, Mouse, Zebrafish (Danio rerio), Cow and Hamster samples.

Quick Overview for RAB3A antibody (AA 2-14) (ABIN1742211)

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-RAB3A Antibody

Binding Specificity: AA 2-14

Purpose: Rabbit anti-Rab3a antiserum

Specificity: Specific for Rab 3a.

Cross-Reactivity (Details): Reacts with: human (P20336), rat (P63012), mouse (P63011), hamster, cow, zebrafish. Other species not tested yet.

Purification: antiserum

Immunogen: Synthetic peptide corresponding to AA 2 to 14 from rat Rab3a (UniProt Id: P63012)

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:1 : 1000, IHC:1 : 500 up to 1 : 1000, IHC-P:not tested yet ELISA:yes

Comment:

ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

References for anti-RAB3A antibody (ABIN1742211)

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Rastaldi, Armelloni, Berra, Li, Pesaresi, Poczewski, Langer, Kerjaschki, Henger, Blattner, Kretzler, Wanke, DAmico: "Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a." in: The American journal of pathology, Vol. 163, Issue 3, pp. 889-99, (2003) (PubMed).

Schlüter, Khvotchev, Jahn, Südhof: "Localization versus function of Rab3 proteins. Evidence for a common regulatory role in controlling fusion." in: The Journal of biological chemistry, Vol. 277, Issue 43, pp. 40919-29, (2002) (PubMed).

Von Kriegstein, Schmitz, Link, Südhof: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).

Target Details for RAB3A

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Alternative Name: Rab3a

Background:

Synonyms: Rab3a, Rab3

Antigen Information: Rab3 proteins are the major Rab isoforms in brain and are highly enriched on synaptic vesicles

UniProt: P63012

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport