Syntaxin 4 antibody (AA 1-273)

Catalog No. ABIN1742221

This Rabbit Polyclonal antibody specifically detects Syntaxin 4 in WB, IP and ICC. It exhibits reactivity toward Human, Rat, Mouse and Hamster and has been mentioned in 6+ publications.

Quick Overview for Syntaxin 4 antibody (AA 1-273) (ABIN1742221)

Target: Syntaxin 4 (STX4)

Reactivity: Human, Rat, Mouse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syntaxin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Syntaxin 4 Antibody

Binding Specificity: AA 1-273

Purpose: Rabbit anti-Syntaxin4 antiserum

Specificity: Specific for syntaxin 4.

Cross-Reactivity (Details): Reacts with: human (Q12846), rat (Q08850), mouse (P70452), hamster. Other species not tested yet.

Purification: antiserum

Immunogen: Recombinant protein corresponding to AA 1 to 273 from rat Syntaxin4 (UniProt Id: Q08850)

Application Details

Application Notes: WB:1 : 1000 up to 1 : 5000 , IP:yes ICC:1 : 100 up to 1 : 500, IHC:not recommended IHC-P:not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

References for anti-Syntaxin 4 antibody (ABIN1742221)

Arnold, Adhikari, Kang, Xu 徐昊: "Munc18a clusters SNARE-bearing liposomes prior to trans-SNARE zippering." in: The Biochemical journal, Vol. 474, Issue 19, pp. 3339-3354, (2017) (PubMed).

Arasaki, Roy: "Legionella pneumophila promotes functional interactions between plasma membrane syntaxins and Sec22b." in: Traffic (Copenhagen, Denmark), Vol. 11, Issue 5, pp. 587-600, (2010) (PubMed).

Bajohrs, Darios, Peak-Chew, Davletov: "Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell." in: The Biochemical journal, Vol. 392, Issue Pt 2, pp. 283-9, (2005) (PubMed).

Li, Alexander, Schwartz: "Syntaxin isoform specificity in the regulation of renal H+-ATPase exocytosis." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 19791-7, (2003) (PubMed).

Toyomura, Murata, Yamamoto, Oka, Sun-Wada, Wada, Futai: "From lysosomes to the plasma membrane: localization of vacuolar-type H+ -ATPase with the a3 isoform during osteoclast differentiation." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 22023-30, (2003) (PubMed).

Riedel, Antonin, Fernandez-Chacon, Alvarez de Toledo, Jo, Geppert, Valentijn, Valentijn, Jamieson, Südhof, Jahn: "Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6487-97, (2002) (PubMed).

Target Details for Syntaxin 4

Target: Syntaxin 4 (STX4)

Alternative Name: Syntaxin4

Background:

Synonyms: stx4, stx 4, SNARES

Antigen Information: Syntaxin4 is an ubiquitous plasma membrane protein involved in fusion of vesicles with the plasma membrane

UniProt: Q08850

Pathways: Synaptic Vesicle Exocytosis