SNAP alpha/beta antibody (AA 1-295)

Catalog No. ABIN1742242

This Mouse Monoclonal antibody specifically detects SNAP alpha/beta in WB, ICC and IP. It exhibits reactivity toward Human, Mouse, Rat and Zebrafish (Danio rerio). It has been mentioned in 3+ publications

Quick Overview for SNAP alpha/beta antibody (AA 1-295) (ABIN1742242)

Target: SNAP alpha/beta

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNAP alpha/beta antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 77-2

Product Details anti-SNAP alpha/beta Antibody

Binding Specificity: AA 1-295

Purpose: Mouse anti-alpha/beta SNAP IgG

Specificity: Specific for alpha- and beta SNAP

Cross-Reactivity (Details): Reacts with: human (P54920, P60880), rat (P54921, P60881), mouse (Q9DB05, P28663), zebrafish. Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 1 to 295 from rat alpha SNAP (UniProt Id: P54921)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 1000 up to 1 : 10000 IHC-P:not tested yet

Comment:

The antibody does not immunoprecipitate the 20 S SNARE-complex.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!

References for anti-SNAP alpha/beta antibody (ABIN1742242)

de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Hanson, Otto, Barton, Jahn: "The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16955-61, (1995) (PubMed).

Target Details for SNAP alpha/beta

Target: SNAP alpha/beta

Background:

Synonyms: SNARE associated, alpha SNAP, beta SNAP, alpha-SNAP, beta-SNAP

Antigen Information: alpha/beta SNAP are peripheral membrane proteins required for membrane traffic

UniProt: P54921