Amphiphysin antibody (AA 2-15)

Catalog No. ABIN1742262

This anti-Amphiphysin antibody is a Rabbit Polyclonal antibody detecting Amphiphysin in WB, IHC, IP and ICC. Suitable for Human, Mouse, Rat, Cow, Hamster and Chicken. This Primary Antibody has been cited in 9+ publications.

Quick Overview for Amphiphysin antibody (AA 2-15) (ABIN1742262)

Target: Amphiphysin (AMPH)

Reactivity: Human, Mouse, Rat, Cow, Hamster, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amphiphysin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Amphiphysin Antibody

Binding Specificity: AA 2-15

Purpose: Rabbit anti-Amphiphysin antiserum

Specificity: Specific for amphiphysin.

Cross-Reactivity (Details): Reacts with: human (P49418), rat (O08838), mouse (Q7TQF7), hamster, cow, chicken. Other species not tested yet.

Purification: antiserum

Immunogen: Synthetic peptide corresponding to AA 2 to 15 from rat Amphiphysin (UniProt Id: O08838)

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:1 : 500, IHC:1 : 400, IHC-P:not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

References for anti-Amphiphysin antibody (ABIN1742262)

Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

Neef, Jung, Wong, Reuter, Pangrsic, Chakrabarti, Kügler, Lenz, Nouvian, Boumil, Frankel, Wichmann, Moser: "Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 3, pp. 705-16, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

von Kleist, Stahlschmidt, Bulut, Gromova, Puchkov, Robertson, MacGregor, Tomilin, Tomlin, Pechstein, Chau, Chircop, Sakoff, von Kries, Saenger, Kräusslich, Shupliakov, Robinson, McCluskey, Haucke: "Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition." in: Cell, Vol. 146, Issue 3, pp. 471-84, (2011) (PubMed).

Scott, Tabarean, Tang, Cartier, Masliah, Roy: "A pathologic cascade leading to synaptic dysfunction in alpha-synuclein-induced neurodegeneration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 24, pp. 8083-95, (2010) (PubMed).

Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).

Basheer, Brown, Ramesh, Begum, McCarley: "Sleep deprivation-induced protein changes in basal forebrain: implications for synaptic plasticity." in: Journal of neuroscience research, Vol. 82, Issue 5, pp. 650-8, (2005) (PubMed).

Fernández-Chacón, Achiriloaie, Janz, Albanesi, Südhof: "SCAMP1 function in endocytosis." in: The Journal of biological chemistry, Vol. 275, Issue 17, pp. 12752-6, (2000) (PubMed).

Von Kriegstein, Schmitz, Link, Südhof: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).

Target Details for Amphiphysin

Target: Amphiphysin (AMPH)

Alternative Name: Amphiphysin

Background:

Synonyms: AMPH

Antigen Information: Amphiphysin is a nerve-terminal protein that binds clathrin, dynamin, and synaptojanin

UniProt: O08838