NSF antibody (AA 733-744)

Catalog No. ABIN1742270

The Rabbit Polyclonal anti-NSF antibody has been validated for WB, ELISA, IP, IHC and ICC. It is suitable to detect NSF in samples from Human, Rat, Mouse and Hamster. There are 5+ publications available.

Quick Overview for NSF antibody (AA 733-744) (ABIN1742270)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity: Human, Rat, Mouse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-NSF Antibody

Binding Specificity: AA 733-744

Purpose: Rabbit anti-NSF antiserum

Specificity: Specific for NSF.

Cross-Reactivity (Details): Reacts with: human (P46459), rat (Q9QUL6), mouse (P46460), hamster. Other species not tested yet.

Purification: antiserum

Immunogen: Synthetic peptide corresponding to AA 733 to 744 from rat NSF (UniProt Id: Q9QUL6)

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:1 : 100 up to 1 : 1000, IHC:1 : 200, IHC-P:not tested yet ELISA:yes

Comment:

ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742269 as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

References for anti-NSF antibody (ABIN1742270)

de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Tian, Wu, Unzicker, Lu, Cai, Li, Schirra, Matti, Stevens, Deng, Rettig, Sheng: "The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 45, pp. 10546-55, (2005) (PubMed).

Tailleux, Neyrolles, Honoré-Bouakline, Perret, Sanchez, Abastado, Lagrange, Gluckman, Rosenzwajg, Herrmann: "Constrained intracellular survival of Mycobacterium tuberculosis in human dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 4, pp. 1939-48, (2003) (PubMed).

Michaut, Tomes, De Blas, Yunes, Mayorga: "Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 18, pp. 9996-10001, (2000) (PubMed).

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: NSF

Background:

Synonyms: SNARE associated, N-ethylmaleimide-sensitive factor, NEM-sensitive fusion protein, SKD2

Antigen Information: NSF is a trimeric ATPase required for membrane fusion during vesicular transport

UniProt: Q9QUL6