NSF antibody (AA 733-744)

Catalog No. ABIN1742270

The Rabbit Polyclonal anti-NSF antibody has been validated for WB, ELISA, IP, IHC and ICC. It is suitable to detect NSF in samples from Human, Rat, Mouse and Hamster. There are 5+ publications available.

Quick Overview for NSF antibody (AA 733-744) (ABIN1742270)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity: Human, Rat, Mouse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-NSF Antibody

Binding Specificity: AA 733-744

Specificity: Specific for NSF.

Purification: antiserum

Immunogen: Synthetic peptide REEGASPLDFD (aa 733-744 in rat NSF) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 100 up to 1 : 1000
IHC: 1 : 200

Comment:

ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742269 as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-NSF antibody (ABIN1742270)

de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Tian, Wu, Unzicker, Lu, Cai, Li, Schirra, Matti, Stevens, Deng, Rettig, Sheng: "The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 45, pp. 10546-55, (2005) (PubMed).

Tailleux, Neyrolles, Honoré-Bouakline, Perret, Sanchez, Abastado, Lagrange, Gluckman, Rosenzwajg, Herrmann: "Constrained intracellular survival of Mycobacterium tuberculosis in human dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 4, pp. 1939-48, (2003) (PubMed).

Michaut, Tomes, De Blas, Yunes, Mayorga: "Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 18, pp. 9996-10001, (2000) (PubMed).

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: NSF