UNC13A/Munc13-1 antibody (AA 3-317)

Catalog No. ABIN1742276

The Mouse Monoclonal anti-UNC13A/Munc13-1 antibody has been validated for WB and IP. It is suitable to detect UNC13A/Munc13-1 in samples from Mouse, Rat and Zebrafish (Danio rerio). There are 6+ publications available.

Quick Overview for UNC13A/Munc13-1 antibody (AA 3-317) (ABIN1742276)

Target: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Reactivity: Mouse, Rat, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This UNC13A/Munc13-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Grade: KO Validated

Clone: 266B1

Product Details anti-UNC13A/Munc13-1 Antibody

Binding Specificity: AA 3-317

Purpose: Mouse anti-Munc13-1 IgG

Specificity: Specific for munc 13-1.

Cross-Reactivity (Details): Reacts with: rat (Q62768), mouse (Q4KUS2), zebrafish. Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)

Isotype: IgG2b

Application Details

Application Notes: WB:1 : 500 up to 1 : 5000 , IP:yes ICC:not tested yet IHC:not tested yet IHC-P:not tested yet ELISA:

Comment:

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: limited to methanol fixation. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-UNC13A/Munc13-1 antibody (ABIN1742276)

Kaeser-Woo, Younts, Yang, Zhou, Wu, Castillo, Südhof: "Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 23, pp. 9769-80, (2013) (PubMed).

Dudenhöffer-Pfeifer, Schirra, Pattu, Halimani, Maier-Peuschel, Marshall, Matti, Becherer, Dirks, Jung, Lipp, Hoth, Sester, Krause, Rettig: "Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes." in: Traffic (Copenhagen, Denmark), Vol. 14, Issue 7, pp. 798-809, (2013) (PubMed).

Maas, Torres, Altrock, Leal-Ortiz, Wagh, Terry-Lorenzo, Fejtova, Gundelfinger, Ziv, Garner: "Formation of Golgi-derived active zone precursor vesicles." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 32, pp. 11095-108, (2012) (PubMed).

Martín, Bartolomé-Martín, Torres, Sánchez-Prieto: "Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals." in: Journal of neurochemistry, Vol. 116, Issue 4, pp. 476-85, (2011) (PubMed).

Martín, Durroux, Ciruela, Torres, Pin, Sánchez-Prieto: "The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17907-17, (2010) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Target Details for UNC13A/Munc13-1

Target: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Alternative Name: Munc13-1

Background:

Synonyms: Munc13-1, Munc13, Protein unc-13 homolog A, Unc13a, Unc13h1, Brain, Hippocampus, Cerebellum, Olfactory bulb, Cortex, Striatum

Antigen Information: K.O. Munc13 proteins are the mammalian homologues of the C. elegans unc-13 gene essential for exocytosis

UniProt: Q62768

Pathways: Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis