UNC13A/Munc13-1 antibody (AA 3-317)

Catalog No. ABIN1742276

The Mouse Monoclonal anti-UNC13A/Munc13-1 antibody has been validated for WB, ELISA and IP. It is suitable to detect UNC13A/Munc13-1 in samples from Mouse, Rat and Zebrafish (Danio rerio). There are 6+ publications available.

Quick Overview for UNC13A/Munc13-1 antibody (AA 3-317) (ABIN1742276)

Target: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Reactivity: Mouse, Rat, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This UNC13A/Munc13-1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 266B1

Product Details anti-UNC13A/Munc13-1 Antibody

Binding Specificity: AA 3-317

Specificity: Specific for munc 13-1.

Purification: purified IgG

Immunogen: Recombinant rat munc 13-1 (aa 3-317).

Isotype: IgG2b

Application Details

Application Notes: WB: 1 : 500 up to 1 : 5000 (AP staining)
ICC: limited
IHC: not tested yet

Comment:

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: limited to methanol fixation. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-UNC13A/Munc13-1 antibody (ABIN1742276)

Kaeser-Woo, Younts, Yang, Zhou, Wu, Castillo, Südhof: "Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 23, pp. 9769-80, (2013) (PubMed).

Dudenhöffer-Pfeifer, Schirra, Pattu, Halimani, Maier-Peuschel, Marshall, Matti, Becherer, Dirks, Jung, Lipp, Hoth, Sester, Krause, Rettig: "Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes." in: Traffic (Copenhagen, Denmark), Vol. 14, Issue 7, pp. 798-809, (2013) (PubMed).

Maas, Torres, Altrock, Leal-Ortiz, Wagh, Terry-Lorenzo, Fejtova, Gundelfinger, Ziv, Garner: "Formation of Golgi-derived active zone precursor vesicles." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 32, pp. 11095-108, (2012) (PubMed).

Martín, Bartolomé-Martín, Torres, Sánchez-Prieto: "Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals." in: Journal of neurochemistry, Vol. 116, Issue 4, pp. 476-85, (2011) (PubMed).

Martín, Durroux, Ciruela, Torres, Pin, Sánchez-Prieto: "The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17907-17, (2010) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Target Details for UNC13A/Munc13-1

Target: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Alternative Name: Munc 13-1

Pathways: Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis