APP antibody (C-Term)

Catalog No. ABIN1742281

This Rabbit Polyclonal antibody specifically detects APP in WB, IHC and ICC. It exhibits reactivity toward Mouse, Rat, Chicken and Frogand has been mentioned in 1 publication.

Quick Overview for APP antibody (C-Term) (ABIN1742281)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Mouse, Rat, Chicken, Frog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-APP Antibody

Binding Specificity: C-Term

Specificity: Specific for APP.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide GYENPTYKFFEQMQN corresponding to the C-terminus of APP coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 500 up to 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC-P: 1 : 2000

Comment:

IHC/IHC-P: The affinity purified antibody is highly recommended.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-APP antibody (ABIN1742281)

Groemer, Thiel, Holt, Riedel, Hua, Hüve, Wilhelm, Klingauf: "Amyloid precursor protein is trafficked and secreted via synaptic vesicles." in: PLoS ONE, Vol. 6, Issue 4, pp. e18754, (2011) (PubMed).

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: APP

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour