Alpha, beta Synuclein (AA 2-25) antibody

Catalog No. ABIN1742282

There are 7+ publications for this product available. The Rabbit Polyclonal anti-Alpha, beta Synuclein antibody is suitable to detect Alpha, beta Synuclein in samples from Human, Mouse, Rat and Zebrafish (Danio rerio). It has been validated for WB, ICC, IHC, IHC (p) and IP.

Quick Overview for Alpha, beta Synuclein (AA 2-25) antibody (ABIN1742282)

Target: Alpha, beta Synuclein

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details

Binding Specificity: AA 2-25

Purpose: Rabbit anti-alpha/beta Synuclein antiserum

Specificity: Recognizes alpha synuclein and beta synuclein.

Cross-Reactivity (Details): Reacts with: rat (P37377, Q63754), mouse (O55042, Q91ZZ3), zebrafish, human (P37840, Q16143). Other species not tested yet.

Purification: antiserum

Immunogen: Synthetic peptide corresponding to AA 2 to 25 from human alpha-Synuclein (UniProt Id: P37840)

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:1 : 500, IHC:1 : 500, IHC-P:1 : 1000 up to 1 : 2000,

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

References

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Siebert, Kahle, Kramer, Isik, Schlüter, Schulz-Schaeffer, Brück: "Over-expression of alpha-synuclein in the nervous system enhances axonal degeneration after peripheral nerve lesion in a transgenic mouse strain." in: Journal of neurochemistry, Vol. 114, Issue 4, pp. 1007-18, (2010) (PubMed).

Tong, Wong, Guttman, Ang, Forno, Shimadzu, Rajput, Muenter, Kish, Hornykiewicz, Furukawa: "Brain alpha-synuclein accumulation in multiple system atrophy, Parkinson's disease and progressive supranuclear palsy: a comparative investigation." in: Brain : a journal of neurology, Vol. 133, Issue Pt 1, pp. 172-88, (2010) (PubMed).

Cole, Dieuliis, Leo, Mitchell, Nussbaum: "Mitochondrial translocation of alpha-synuclein is promoted by intracellular acidification." in: Experimental cell research, Vol. 314, Issue 10, pp. 2076-89, (2008) (PubMed).

Colapinto, Mila, Giraudo, Stefanazzi, Molteni, Rossetti, Bergamasco, Lopiano, Fasano: "alpha-Synuclein protects SH-SY5Y cells from dopamine toxicity." in: Biochemical and biophysical research communications, Vol. 349, Issue 4, pp. 1294-300, (2006) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Steinacker, Schwarz, Reim, Brechlin, Jahn, Kratzin, Aitken, Wiltfang, Aguzzi, Bahn, Baxter, Brose, Otto: "Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein." in: Molecular and cellular biology, Vol. 25, Issue 4, pp. 1339-46, (2005) (PubMed).

Target Details

Target: Alpha, beta Synuclein

Alternative Name: alpha/beta Synuclein

Background:

Synonyms: alpha/beta Synuclein, SNCA, SNCB, NACP, Synuclein

Antigen Information: Synucleins are involved in Parkinson's and Alzheimer's Disease

UniProt: P37840