Neuroligin (NEUROLIGIN) (AA 1-695) antibody

Catalog No. ABIN1742285

The Mouse Monoclonal anti- antibody has been validated for IP and WB. It is suitable to detect in samples from Mouse and Rat. There are 6+ publications available.

Quick Overview for Neuroligin (NEUROLIGIN) (AA 1-695) antibody (ABIN1742285)

Target: Neuroligin (NEUROLIGIN)

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Grade: KO Validated

Clone: 4F9

Product Details

Binding Specificity: AA 1-695

Purpose: Mouse anti-Neuroligin1/2/3/4 IgG

Specificity: Recognizes rat and mouse neuroligins 1-3 and rat neuroligin 4.

Cross-Reactivity (Details): Reacts with: rat (Q62765, Q62888, Q62889, ), mouse (Q99K10, Q69ZK9, Q8BYM5, B0F2B4). Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 1 to 695 from rat Neuroligin1 (UniProt Id: Q62765)

Isotype: IgG2a

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:not recommended IHC:not recommended IHC-P:not tested yet

Comment:

ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References

Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." in: Frontiers in cellular neuroscience, Vol. 9, pp. 24, (2015) (PubMed).

Fujita-Jimbo, Tanabe, Yu, Kojima, Mori, Li, Iwamoto, Yamagata, Momoi, Momoi: "The association of GPR85 with PSD-95-neuroligin complex and autism spectrum disorder: a molecular analysis." in: Molecular autism, Vol. 6, pp. 17, (2015) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Bottos, Destro, Rissone, Graziano, Cordara, Assenzio, Cera, Mascia, Bussolino, Arese: "The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 49, pp. 20782-7, (2010) (PubMed).

De Jaco, Comoletti, Kovarik, Gaietta, Radic, Lockridge, Ellisman, Taylor: "A mutation linked with autism reveals a common mechanism of endoplasmic reticulum retention for the alpha,beta-hydrolase fold protein family." in: The Journal of biological chemistry, Vol. 281, Issue 14, pp. 9667-76, (2006) (PubMed).

Chih, Engelman, Scheiffele: "Control of excitatory and inhibitory synapse formation by neuroligins." in: Science (New York, N.Y.), Vol. 307, Issue 5713, pp. 1324-8, (2005) (PubMed).

Target Details

Target: Neuroligin (NEUROLIGIN)

Alternative Name: Neuroligin

Background:

Synonyms: Neuroligin1, Neuroligin2, Neuroligin3, Synaptic Adhesion Proteins, Neuroligin 1, Neuroligin 2, Neuroligin 3, Neuroligin 4, Neuroligin4

Antigen Information: K.O. Neuroligins are postsynaptic cell adhesion molecules

UniProt: Q62765