Piccolo antibody (Center)

Catalog No. ABIN1742313

This anti-Piccolo antibody is a Guinea Pig Polyclonal antibody detecting Piccolo in WB, IHC, ICC, IHC (p) and IP. Suitable for Rat and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for Piccolo antibody (Center) (ABIN1742313)

Target: Piccolo (PIC)

Reactivity: Rat, Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Piccolo antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Grade: KO Validated

Product Details anti-Piccolo Antibody

Binding Specificity: Center

Purpose: anti-Piccolo antiserum

Specificity: Specific for piccolo.

Cross-Reactivity (Details): Reacts with: rat (Q9JKS6), mouse (Q9QYX7). Other species not tested yet.

Purification: antiserum

Immunogen: Recombinant protein corresponding to a central region of rat piccolo (UniProt Id: Q9JKS6)

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:1 : 500 up to 1 : 1000, IHC:1 : 200, IHC-P:1 : 500,

Comment:

WB: Due to its large size, piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed. This antibody detects an additional band of ~65 kDa.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O, then aliquot and store at -20 °C until use.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

References for anti-Piccolo antibody (ABIN1742313)

Babai, Sendelbeck, Regus-Leidig, Fuchs, Mertins, Reim, Brose, Feigenspan, Brandstätter: "Functional Roles of Complexin3 and Complexin4 at Mouse Photoreceptor Ribbon Synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 25, pp. 6651-67, (2016) (PubMed).

Target Details for Piccolo

Target: Piccolo (PIC)

Alternative Name: Piccolo

Background:

Synonyms: Aczonin, Pclo, ACZ

Antigen Information: K.O. Piccolo is a large multi-domain protein of the presynaptic active zone

UniProt: Q9JKS6