ERC2 antibody (AA 655-670)

Catalog No. ABIN1742314

This Rabbit Polyclonal antibody specifically detects ERC2 in WB. It exhibits reactivity toward Mouse, Rat and Hamster and has been mentioned in 3+ publications.

Quick Overview for ERC2 antibody (AA 655-670) (ABIN1742314)

Target: ERC2 (ELKS/RAB6-Interacting/CAST Family Member 2 (ERC2))

Reactivity: Mouse, Rat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERC2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERC2 Antibody

Binding Specificity: AA 655-670

Specificity: Specific for ERC 2.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide CDIEDDSRMNPEFADRLK (aa 655-670 in rat ERC 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 100 up to 1 : 2000 (AP staining)
IP: not tested yet
ICC: not recommended
IHC: not recommended

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in TBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-ERC2 antibody (ABIN1742314)

Uthaiah, Hudspeth: "Molecular anatomy of the hair cell's ribbon synapse." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 37, pp. 12387-99, (2010) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

tom Dieck, Altrock, Kessels, Qualmann, Regus, Brauner, Fejtová, Bracko, Gundelfinger, Brandstätter: "Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex." in: The Journal of cell biology, Vol. 168, Issue 5, pp. 825-36, (2005) (PubMed).

Target Details for ERC2

Target: ERC2 (ELKS/RAB6-Interacting/CAST Family Member 2 (ERC2))

Alternative Name: ERC 2

Background: Synonyms: ELKS 2, CAST 1