Synaptojanin 1 antibody (AA 1292-1308)

Catalog No. ABIN1742316

This anti-Synaptojanin 1 antibody is a Rabbit Polyclonal antibody detecting Synaptojanin 1 in WB. Suitable for Rat, Mouse and Hamster. This Primary Antibody has been cited in 7+ publications.

Quick Overview for Synaptojanin 1 antibody (AA 1292-1308) (ABIN1742316)

Target: Synaptojanin 1 (SYNJ1)

Reactivity: Rat, Mouse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptojanin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Synaptojanin 1 Antibody

Binding Specificity: AA 1292-1308

Purpose: anti-Synaptojanin1

Specificity: Recognizes all isoforms of synaptojanin 1.

Cross-Reactivity (Details): Reacts with: rat (Q62910), mouse (Q8CHC4), hamster. Other species not tested yet.

Purification: affinity purified

Immunogen: Synthetic peptide corresponding to AA 1292 to 1308 from rat Synaptojanin1 (UniProt Id: Q62910-1)

Application Details

Application Notes: WB:1 : 200 up to 1 : 2000 IHC-P:not tested yet

Comment:

Running SDS-PAGE with non-boiled samples gives stronger signals.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-Synaptojanin 1 antibody (ABIN1742316)

Cliburn, Dunn, Stout, Hoffman, Lohr, Bernstein, Winokur, Burkett, Schmitz, Caudle, Miller: "Immunochemical localization of vesicular monoamine transporter 2 (VMAT2) in mouse brain." in: Journal of chemical neuroanatomy, (2016) (PubMed).

Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Park, Kim, Lee, Park, Park, Sun, Kim, Chang: "SNX18 shares a redundant role with SNX9 and modulates endocytic trafficking at the plasma membrane." in: Journal of cell science, Vol. 123, Issue Pt 10, pp. 1742-50, (2010) (PubMed).

Uthaiah, Hudspeth: "Molecular anatomy of the hair cell's ribbon synapse." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 37, pp. 12387-99, (2010) (PubMed).

Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).

Shin, Lee, Ahn, Kim, Ahn, YongPark, Chang: "Sorting nexin 9 interacts with dynamin 1 and N-WASP and coordinates synaptic vesicle endocytosis." in: The Journal of biological chemistry, Vol. 282, Issue 39, pp. 28939-50, (2007) (PubMed).

Target Details for Synaptojanin 1

Target: Synaptojanin 1 (SYNJ1)

Alternative Name: Synaptojanin1

Background: Synj1

UniProt: Q62910

Pathways: Inositol Metabolic Process, Synaptic Vesicle Exocytosis