Gephyrin antibody (C-Term)

Catalog No. ABIN1742320

This Rabbit Polyclonal antibody specifically detects Gephyrin in WB and IP. It exhibits reactivity toward Mouse and Rat.

Quick Overview for Gephyrin antibody (C-Term) (ABIN1742320)

Target: Gephyrin (GPHN)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Gephyrin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Gephyrin Antibody

Binding Specificity: AA 294-736, C-Term

Specificity: Specific for gephyrin.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein containing the C-terminal part of rat gephyrin (aa 294-736).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)

Comment:

IP: For most effective IP use the solubilization protocol described in this ELISA protocol. Consider that protein-protein interaction may be affected.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Target Details for Gephyrin

Target: Gephyrin (GPHN)

Alternative Name: Gephyrin

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development