Arc antibody (AA 1-214)

Catalog No. ABIN1742333

The Mouse Monoclonal anti-Arc antibody has been validated for ELISA and IP. It is suitable to detect Arc in samples from Rat and Mouse. There are 5+ publications available.

Quick Overview for Arc antibody (AA 1-214) (ABIN1742333)

Target: Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Arc antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP)

Clone: 48B2

Product Details anti-Arc Antibody

Binding Specificity: AA 1-214

Specificity: Specific for Arc.

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Strep-Tag® fusion protein of full-length mouse arc. Epitope Binds within first 214 aa.

Isotype: IgG2b

Application Details

Application Notes: WB: not recommended
ICC: not tested yet
IHC: not tested yet

Comment:

ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Arc antibody (ABIN1742333)

Izumi, Ishimoto, Yamamoto, Mori: "Application of hairless mouse strain to bioluminescence imaging of Arc expression in mouse brain." in: BMC neuroscience, Vol. 18, Issue 1, pp. 18, (2017) (PubMed).

Gerace, Landucci, Totti, Bani, Guasti, Baronti, Moroni, Mannaioni, Pellegrini-Giampietro: "Ethanol Toxicity During Brain Development: Alterations of Excitatory Synaptic Transmission in Immature Organotypic Hippocampal Slice Cultures." in: Alcoholism, clinical and experimental research, Vol. 40, Issue 4, pp. 706-16, (2016) (PubMed).

Lee, Kang, Kim, Yoon, Kim, Moon: "Enhanced expression of immediate-early genes in mouse hippocampus after trimethyltin treatment." in: Acta histochemica, (2016) (PubMed).

Alvarez-Dieppa, Griffin, Cavalier, McIntyre: "Vagus Nerve Stimulation Enhances Extinction of Conditioned Fear in Rats and Modulates Arc Protein, CaMKII, and GluN2B-Containing NMDA Receptors in the Basolateral Amygdala." in: Neural plasticity, Vol. 2016, pp. 4273280, (2016) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Target Details for Arc

Target: Arc (Activity-Regulated Cytoskeleton-Associated Protein (Arc))

Alternative Name: Arc

Background: Synonyms: Arg 3.1