SHANK3 antibody (AA 1055-1616)

Catalog No. ABIN1742347

Product Details anti-SHANK3 Antibody

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Binding Specificity: AA 1055-1616

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHANK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Specific for shank 3.

Purification: antiserum

Immunogen: Recombinant protein (aa 1055-1616 of rat shank 3).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500

Comment:

IHC: For best results the antibody requires a mild fixation protocol (2 % PFA in sodium acetate buffer, pH 6).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SHANK3 antibody (ABIN1742347)

Tao-Cheng, Toy, Winters, Reese, Dosemeci: "Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses." in: PLoS ONE, Vol. 11, Issue 5, pp. e0153979, (2016) (PubMed).

Wei, Ma, Ding, Jin, Liu, Chang, Hu, Yu: "Reduced Glutamate Release in Adult BTBR Mouse Model of Autism Spectrum Disorder." in: Neurochemical research, Vol. 41, Issue 11, pp. 3129-3137, (2016) (PubMed).

Wei, Ma, Liu, Ding, Jin, Wang, Hu, Yu: "Inhibition of IL-6 trans-signaling in the brain increases sociability in the BTBR mouse model of autism." in: Biochimica et biophysica acta, Vol. 1862, Issue 10, pp. 1918-25, (2016) (PubMed).

Hagmeyer, Mangus, Boeckers, Grabrucker: "Effects of trace metal profiles characteristic for autism on synapses in cultured neurons." in: Neural plasticity, Vol. 2015, pp. 985083, (2015) (PubMed).

Target Details for SHANK3

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Alternative Name: Shank 3 (SHANK3 Products)

Background: Synonyms: ProSAP2, SPANK2

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity