RAB27A antibody (AA 1-221)

Catalog No. ABIN1742354

The Rabbit Polyclonal anti-RAB27A antibody has been validated for WB, IP and IHC (p). It is suitable to detect RAB27A in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for RAB27A antibody (AA 1-221) (ABIN1742354)

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB27A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-RAB27A Antibody

Binding Specificity: AA 1-221

Purpose: Rabbit anti-Rab27A antibody

Specificity: Specific for rab 27A

Cross-Reactivity (Details): Reacts with: rat (P23640), mouse (Q9ERI2), human (P51159). Other species not tested yet.

Purification: affinity purified

Immunogen: Recombinant protein corresponding to AA 1 to 221 from rat Rab27A (UniProt Id: P23640)

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:not tested yet IHC:not tested yet IHC-P:1 : 2000,

Comment:

Only the non-conserved C-terminal part of the protein was used for affinity purification to achieve specificity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin was added for stabilization.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin was added for stabilization. Do not freeze!

References for anti-RAB27A antibody (ABIN1742354)

Hou, Ernst, Stuenkel, Lentz, Williams: "Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion." in: PLoS ONE, Vol. 10, Issue 5, pp. e0125596, (2015) (PubMed).

Lovis, Roggli, Laybutt, Gattesco, Yang, Widmann, Abderrahmani, Regazzi: "Alterations in microRNA expression contribute to fatty acid-induced pancreatic beta-cell dysfunction." in: Diabetes, Vol. 57, Issue 10, pp. 2728-36, (2008) (PubMed).

Target Details for RAB27A

Target: RAB27A (RAB27A, Member RAS Oncogene Family (RAB27A))

Alternative Name: Rab27A

Background:

Synonyms: Rab27, pancreas, insulin granules, secretory vesicles, pancreatic islands

Antigen Information: K.O. Rab27 proteins are members of the Rab family responsible for melanosomal transport and hormone secretion

UniProt: P23640

Pathways: Peptide Hormone Metabolism