GFAP antibody (Biotin)

Catalog No. ABIN1742362

This Mouse Monoclonal antibody specifically detects GFAP in WB, ELISA and IP. It exhibits reactivity toward Human, Rat, Mouse and Cowand has been mentioned in 1 publication.

Quick Overview for GFAP antibody (Biotin) (ABIN1742362)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Grade: KO Validated

Clone: 186C6

Product Details anti-GFAP Antibody (Biotin)

Purpose: Mouse anti-GFAP IgG

Cross-Reactivity (Details): Reacts with: human (P14136), rat (P47819), mouse (P03995), cow. Other species not tested yet.

Characteristics: labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

Purification: purified IgG

Immunogen: full-length recombinant human GFAP (UniProt Id: P14136) Epitop AA 1 to 15 from human GFAP (UniProt Id: P14136)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:not recommended IHC:not recommended IHC-P:not tested yet ELISA:yes

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS

Preservative: Without preservative

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!

References for anti-GFAP antibody (Biotin) (ABIN1742362)

Ganau, Bosco, Palma, Corvaglia, Parisse, Fruk, Beltrami, Cesselli, Casalis, Scoles: "A DNA-based nano-immunoassay for the label-free detection of glial fibrillary acidic protein in multicell lysates." in: Nanomedicine : nanotechnology, biology, and medicine, Vol. 11, Issue 2, pp. 293-300, (2015) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background:

Synonyms: Glial fibrillary acidic protein, Neuroinflamm-Astrocyt

Antigen Information: K.O. GFAP is an astrocyte-specific type-III intermediate filament protein

UniProt: P14136