Neurexin 1 antibody (AA 1328-1421)

Catalog No. ABIN1742366

The Rabbit Polyclonal anti-Neurexin 1 antibody has been validated for WB. It is suitable to detect Neurexin 1 in samples from Rat. There are 2+ publications available.

Quick Overview for Neurexin 1 antibody (AA 1328-1421) (ABIN1742366)

Target: Neurexin 1 (NRXN1)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurexin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Neurexin 1 Antibody

Binding Specificity: AA 1328-1421

Specificity: Specific for neurexin 1. The epitope is present in alpha- and beta-neurexin 1.

Cross-Reactivity: Mouse

Purification: Affinity purified with the immunogen. Albumin and azide were added for stabilization.

Immunogen: Recombinant protein corresponding to AA 1328 to 1421 from rat Neurexin1

Application Details

Application Notes: WB: 1 : 500 up to 1 : 1000 (AP staining)
IHC (P): not tested yet
ICC: not recommended
IHC: not recommended

Comment:

WB: Unboiled samples yield stronger signals.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-Neurexin 1 antibody (ABIN1742366)

Bot, Schweizer, Ben Halima, Fraering: "Processing of the synaptic cell adhesion molecule neurexin-3beta by Alzheimer disease alpha- and gamma-secretases." in: The Journal of biological chemistry, Vol. 286, Issue 4, pp. 2762-73, (2011) (PubMed).

Lim, Kwon, Lee, Moon, Jeong, Park, Kim, Park, Lee, Ryu, Yu, Chung, Kim, Myung, Lee: "Synapse formation regulated by protein tyrosine phosphatase receptor T through interaction with cell adhesion molecules and Fyn." in: The EMBO journal, Vol. 28, Issue 22, pp. 3564-78, (2009) (PubMed).

Target Details for Neurexin 1

Target: Neurexin 1 (NRXN1)

Alternative Name: Neurexin 1

Background: Neurexins are receptor-like neuronal cell-surface protein.

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development