VAMP5 antibody (AA 1-70)

Catalog No. ABIN1742369

The Rabbit Polyclonal anti-VAMP5 antibody has been validated for IHC, WB, IHC (p) and ICC. It is suitable to detect VAMP5 in samples from Mouse. There are 4+ publications available.

Quick Overview for VAMP5 antibody (AA 1-70) (ABIN1742369)

Target: VAMP5 (Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAMP5 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-VAMP5 Antibody

Binding Specificity: AA 1-70

Purpose: Rabbit anti-Myobrevin antibody

Specificity: Specific for myobrevin.

Cross-Reactivity (Details): Reacts with: mouse (Q9Z2P8). No signal: rat. Other species not tested yet.

Purification: affinity purified

Immunogen: Recombinant protein corresponding to AA 1 to 70 from mouse Myobrevin (UniProt Id: Q9Z2P8)

Application Details

Application Notes: WB:1 : 1000 up to 1 : 5000 , IP:not tested yet ICC:1 : 1000, IHC:1 : 500 IHC-P:1 : 200,

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin was added for stabilization.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin was added for stabilization. Do not freeze!

References for anti-VAMP5 antibody (ABIN1742369)

Ikezawa, Tajika, Ueno, Murakami, Inoue, Yorifuji: "Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung." in: Developmental dynamics : an official publication of the American Association of Anatomists, (2017) (PubMed).

Sadler, Bryant, Gould: "Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking." in: Molecular biology of the cell, Vol. 26, Issue 3, pp. 530-6, (2015) (PubMed).

Takahashi, Tajika, Khairani, Ueno, Murakami, Yorifuji: "The localization of VAMP5 in skeletal and cardiac muscle." in: Histochemistry and cell biology, Vol. 139, Issue 4, pp. 573-82, (2013) (PubMed).

Rose, Jeppesen, Kiens, Richter: "Effects of contraction on localization of GLUT4 and v-SNARE isoforms in rat skeletal muscle." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 297, Issue 5, pp. R1228-37, (2009) (PubMed).

Target Details for VAMP5

Target: VAMP5 (Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5))

Alternative Name: Myobrevin

Background:

Synonyms: VAMP 5, SNARES

Antigen Information: K.O. Myobrevin or Vamp5 is a VAMP isoform expressed preferentially in skeletal muscle and heart

UniProt: Q9Z2P8