Otoferlin antibody (Isoform 1)

Catalog No. ABIN1742371

This Rabbit Polyclonal antibody specifically detects Otoferlin in IHC. It exhibits reactivity toward Mouseand has been mentioned in 1 publication.

Quick Overview for Otoferlin antibody (Isoform 1) (ABIN1742371)

Target: Otoferlin (OTOF)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Otoferlin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-Otoferlin Antibody

Binding Specificity: AA 196-211, Isoform 1

Specificity: Specific for otoferlin.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide GKTRSHKEEPQRQDEP (aa 196-211 in mouse isoform 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 5000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-Otoferlin antibody (ABIN1742371)

Vogl, Cooper, Neef, Wojcik, Reim, Reisinger, Brose, Rhee, Moser, Wichmann: "Unconventional molecular regulation of synaptic vesicle replenishment in cochlear inner hair cells." in: Journal of cell science, Vol. 128, Issue 4, pp. 638-44, (2015) (PubMed).

Target Details for Otoferlin

Target: Otoferlin (OTOF)

Alternative Name: Otoferlin

Pathways: Sensory Perception of Sound, Synaptic Vesicle Exocytosis