WB: 1 : 1000 (AP staining) ICC: 1 : 100 up to 1 : 500 IHC: 1 : 100 up to 1 : 500
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS, then aliquot and store at -20 °C until use.
Buffer
PBS
Handling Advice
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Storage
-20 °C
Storage Comment
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Choi, Kim, Kim, Lee, Lee, Kho, Sohn, Suh: "Zinc plus cyclo-(His-Pro) promotes hippocampal neurogenesis in rats." in: Neuroscience, Vol. 339, pp. 634-643, (2016) (PubMed).
Ishihara, Fukuda: "Immunohistochemical investigation of the internal structure of the mouse subiculum." in: Neuroscience, Vol. 337, pp. 242-266, (2016) (PubMed).
Kuwahara, Inoue, DAgati, Fujimoto, Eguchi, Saha, Wolozin, Iwatsubo, Abeliovich: "LRRK2 and RAB7L1 coordinately regulate axonal morphology and lysosome integrity in diverse cellular contexts." in: Scientific reports, Vol. 6, pp. 29945, (2016) (PubMed).
Target
Slc30a3
(Solute Carrier Family 30 (Zinc Transporter), Member 3 (Slc30a3))