ChT antibody (C-Term)

Catalog No. ABIN1742430

This anti-ChT antibody is a Mouse Monoclonal antibody detecting ChT in WB, IHC, IP and IHC (p). Suitable for Mouse, Rat and Monkey. This Primary Antibody has been cited in 1 publication.

Quick Overview for ChT antibody (C-Term) (ABIN1742430)

Target: ChT (High Affinity Choline Transporter (ChT))

Reactivity: Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This ChT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Clone: 62-2E8

Product Details anti-ChT Antibody

Binding Specificity: C-Term

Purpose: Mouse anti-ChT IgG

Specificity: Specific for the high affinity choline transporter.

Cross-Reactivity (Details): Reacts with: monkey, rat (Q9JMD7), mouse (Q8BGY9). Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to residues near the C-terminus of rat ChT (UniProt Id: Q9JMD7)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 1000 ,

Comment:

ChT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-ChT antibody (ABIN1742430)

Misawa, Fujigaya, Nishimura, Moriwaki, Okuda, Kawashima, Nakata, Ruggiero, Blakely, Nakatsu, Ohno: "Aberrant trafficking of the high-affinity choline transporter in AP-3-deficient mice." in: The European journal of neuroscience, Vol. 27, Issue 12, pp. 3109-17, (2008) (PubMed).

Target Details for ChT

Target: ChT (High Affinity Choline Transporter (ChT))

Alternative Name: ChT

Background:

Synonyms: Choline transporter, SLC5A7, Cht1, Solute carrier family 5 member 7, Hemicholinium-3-sensitive choline transporter, High affinity choline transporter 1, Choline transporter und cholinergic neurons, Spinal cord, striatum, cholinergic, Striatum, Neuromuscular junction, Brain, Neurotransmitter transporter, Myelencephalon, Aorta, Heart, PONS, Brainstem, Hemicholinium-3-sensitive choline transporter , CHT

Antigen Information: K.O. ChT is a high affinity choline transporter

UniProt: Q9JMD7