TNC antibody

Catalog No. ABIN1742432

The Rat Monoclonal anti-TNC antibody has been validated for WB, IHC and ICC. It is suitable to detect TNC in samples from Rat and Mouse. There are 5+ publications available.

Quick Overview for TNC antibody (ABIN1742432)

Target: TNC (Tenascin C (TNC))

Reactivity: Rat, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 578

Product Details anti-TNC Antibody

Specificity: Specific for tenascin-C splice variants carrying the FNIII D domain.

Purification: hybridoma supernatant

Immunogen: Glycopeptidase F-treated tenascin from mouse brain. Epitope FNIII D domain

Isotype: IgG

Application Details

Application Notes: 1:10.000 recommended dilution

Comment:

Tenascin-C variants detected by this antibody are downregulated during development and hardly detectable in adult animals

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.

Buffer: PBS

Handling Advice: When hybridoma supernatant is reconstituted, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen (-20 °C). Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles.
Prolonged storage at 4 °C is not recommended! Unlike serum, ascites may contain proteases that will ultimately degrade the antibodies. Addition of protease inhibitors helps to slow degradation.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-TNC antibody (ABIN1742432)

Siddiqui, Horvat-Bröcker, Faissner: "The glia-derived extracellular matrix glycoprotein tenascin-C promotes embryonic and postnatal retina axon outgrowth via the alternatively spliced fibronectin type III domain TNfnD." in: Neuron glia biology, Vol. 4, Issue 4, pp. 271-83, (2010) (PubMed).

Rigato, Garwood, Calco, Heck, Faivre-Sarrailh, Faissner: "Tenascin-C promotes neurite outgrowth of embryonic hippocampal neurons through the alternatively spliced fibronectin type III BD domains via activation of the cell adhesion molecule F3/contactin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 15, pp. 6596-609, (2002) (PubMed).

Bartsch, Bartsch, Dörries, Faissner, Weller, Ekblom, Schachner: "Expression of tenascin in the developing and adult cerebellar cortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 12, Issue 3, pp. 736-49, (1992) (PubMed).

Stern, Norris, Bronner-Fraser, Carlson, Faissner, Keynes, Schachner: "J1/tenascin-related molecules are not responsible for the segmented pattern of neural crest cells or motor axons in the chick embryo." in: Development (Cambridge, England), Vol. 107, Issue 2, pp. 309-19, (1990) (PubMed).

Gatchalian, Schachner, Sanes: "Fibroblasts that proliferate near denervated synaptic sites in skeletal muscle synthesize the adhesive molecules tenascin(J1), N-CAM, fibronectin, and a heparan sulfate proteoglycan." in: The Journal of cell biology, Vol. 108, Issue 5, pp. 1873-90, (1989) (PubMed).

Target Details for TNC

Target: TNC (Tenascin C (TNC))

Alternative Name: Tenascin-C

Background: Synonyms: TN-C, J1-200/220, Cytoactin

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development