NCAM 180 (AA 1-1115) antibody

Catalog No. ABIN1742446

This Rat Monoclonal antibody specifically detects NCAM 180 in ICC, IHC and WB. It exhibits reactivity toward Mouse and Rat. It has been mentioned in 3+ publications

Quick Overview for NCAM 180 (AA 1-1115) antibody (ABIN1742446)

Target: NCAM 180

Reactivity: Mouse, Rat

Host: Rat

Clonality: Monoclonal

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)

Clone: 481

Product Details

Binding Specificity: AA 1-1115

Purpose: Rat anti-NCAM180 IgG

Specificity: Specific for NCAM 180.

Cross-Reactivity (Details): Reacts with: rat (P13596), mouse (P13595). Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 1 to 1115 from mouse NCAM180 (UniProt Id: P13595)

Isotype: IgG

Application Details

Application Notes: WB:1 : 1000

Comment:

This antibody lacks the Fc region and has to be used like a Fab fragment. Secondary reagents directed exclusively against the Fc region are not recommended.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!

References

Schuster, Krug, Stalder, Hackel, Gerardy-Schahn, Schachner et al.: "Immunoelectron microscopic localization of the neural recognition molecules L1, NCAM, and its isoform NCAM180, the NCAM-associated polysialic acid, beta1 integrin and the extracellular matrix ..." in: Journal of neurobiology, Vol. 49, Issue 2, pp. 142-58, (2001) (PubMed).

Kramer, Hall, Bleistein, Schachner: "Developmentally regulated masking of an intracellular epitope of the 180 kDa isoform of the neural cell adhesion molecule NCAM." in: Journal of neuroscience research, Vol. 49, Issue 2, pp. 161-75, (1997) (PubMed).

Pollerberg, Sadoul, Goridis, Schachner: "Selective expression of the 180-kD component of the neural cell adhesion molecule N-CAM during development." in: The Journal of cell biology, Vol. 101, Issue 5 Pt 1, pp. 1921-9, (1985) (PubMed).

Target Details

Target: NCAM 180

Alternative Name: NCAM180

Background:

Synonyms: CD 56, NCAM1, N-CAM1, CD56, Synaptic Adhesion Proteins, NCAM180

Antigen Information: NCAM180 is a neural cell adhesion molecule

UniProt: P13595