LRRC32 antibody (AA 296-308)

Catalog No. ABIN1742450

The Mouse Monoclonal anti-LRRC32 antibody has been validated for FACS. It is suitable to detect LRRC32 in samples from Human. There are 3+ publications available.

Quick Overview for LRRC32 antibody (AA 296-308) (ABIN1742450)

Target: LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LRRC32 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 272G6

Product Details anti-LRRC32 Antibody

Binding Specificity: AA 296-308

Purpose: Mouse anti-GARP IgG

Specificity: Specific for GARP.

Cross-Reactivity (Details): Reacts with: human (Q14392). Other species not tested yet.

Purification: purified IgG

Immunogen: Synthetic peptide corresponding to AA 296 to 308 from human GARP (UniProt Id: Q14392)

Isotype: IgG3

Application Details

Application Notes: WB:not recommended IP:not tested yet ICC:not tested yet IHC:not tested yet IHC-P:not tested yet FACS:yes

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Azide was added before lyophilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!

References for anti-LRRC32 antibody (ABIN1742450)

Cuende, Liénart, Dedobbeleer, van der Woning, De Boeck, Stockis, Huygens, Colau, Somja, Delvenne, Hannon, Baron, Dumoutier, Renauld, De Haard, Saunders, Coulie, Lucas: "Monoclonal antibodies against GARP/TGF-β1 complexes inhibit the immunosuppressive activity of human regulatory T cells in vivo." in: Science translational medicine, Vol. 7, Issue 284, pp. 284ra56, (2015) (PubMed).

Rappl, Pabst, Riemann, Schmidt, Wickenhauser, Schütte, Hombach, Seliger, Grohé, Abken: "Regulatory T cells with reduced repressor capacities are extensively amplified in pulmonary sarcoid lesions and sustain granuloma formation." in: Clinical immunology (Orlando, Fla.), Vol. 140, Issue 1, pp. 71-83, (2011) (PubMed).

Probst-Kepper, Geffers, Kröger, Viegas, Erck, Hecht, Lünsdorf, Roubin, Moharregh-Khiabani, Wagner, Ocklenburg, Jeron, Garritsen, Arstila, Kekäläinen, Balling, Hauser, Buer, Weiss: "GARP: a key receptor controlling FOXP3 in human regulatory T cells." in: Journal of cellular and molecular medicine, Vol. 13, Issue 9B, pp. 3343-57, (2010) (PubMed).

Target Details for LRRC32

Target: LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

Alternative Name: GARP

Background:

Synonyms: LRRC 32, CD 109

Antigen Information: GARP is an orphan receptor on Treg-cells

UniProt: Q14392

Pathways: Activated T Cell Proliferation