LRRC32 antibody (AA 296-308)

Catalog No. ABIN1742450

The Mouse Monoclonal anti-LRRC32 antibody has been validated for FACS. It is suitable to detect LRRC32 in samples from Human. There are 3+ publications available.

Quick Overview for LRRC32 antibody (AA 296-308) (ABIN1742450)

Target: LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LRRC32 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 227G6

Product Details anti-LRRC32 Antibody

Binding Specificity: AA 296-308

Specificity: Specific for GARP.

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Synthetic peptide (aa 296-308 of human GARP) coupled to KLH via N-terminal added cysteine.

Isotype: IgG3

Application Details

Application Notes: WB: not recommended
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
FACS: 1 : 100 up to 1 : 1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-LRRC32 antibody (ABIN1742450)

Cuende, Liénart, Dedobbeleer, van der Woning, De Boeck, Stockis, Huygens, Colau, Somja, Delvenne, Hannon, Baron, Dumoutier, Renauld, De Haard, Saunders, Coulie, Lucas: "Monoclonal antibodies against GARP/TGF-β1 complexes inhibit the immunosuppressive activity of human regulatory T cells in vivo." in: Science translational medicine, Vol. 7, Issue 284, pp. 284ra56, (2015) (PubMed).

Rappl, Pabst, Riemann, Schmidt, Wickenhauser, Schütte, Hombach, Seliger, Grohé, Abken: "Regulatory T cells with reduced repressor capacities are extensively amplified in pulmonary sarcoid lesions and sustain granuloma formation." in: Clinical immunology (Orlando, Fla.), Vol. 140, Issue 1, pp. 71-83, (2011) (PubMed).

Probst-Kepper, Geffers, Kröger, Viegas, Erck, Hecht, Lünsdorf, Roubin, Moharregh-Khiabani, Wagner, Ocklenburg, Jeron, Garritsen, Arstila, Kekäläinen, Balling, Hauser, Buer, Weiss: "GARP: a key receptor controlling FOXP3 in human regulatory T cells." in: Journal of cellular and molecular medicine, Vol. 13, Issue 9B, pp. 3343-57, (2010) (PubMed).

Target Details for LRRC32

Target: LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

Alternative Name: GARP

Background: Synonyms: LRRC 32

Pathways: Activated T Cell Proliferation