CHRNB1 antibody

Catalog No. ABIN1742452

This Rat Monoclonal antibody specifically detects CHRNB1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for CHRNB1 antibody (ABIN1742452)

Target: CHRNB1 (Nicotinic Acetylcholine Receptor beta (CHRNB1))

Reactivity: Human, Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This CHRNB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 2-1-155

Product Details anti-CHRNB1 Antibody

Purpose: Rat anti-m-AChR-2 IgG

Specificity: Specific for muscarinic acetylcholine receptor 2.

Cross-Reactivity (Details): Reacts with: human (P08172), rat (P10980), mouse (Q9ERZ4). Other species not tested yet.

Purification: purified IgG

Immunogen: 207 to 388 from human m-AChR-2 (UniProt Id: P08172) Epitop AA 207 to 388 from human m-AChR-2 (UniProt Id: P08172)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 1000 , IP:not tested yet ICC:not tested yet IHC:1 : 100, IHC-P:not tested yet

Comment:

This antibody detects also higher molecular weight bands, depending on the glycosilation state of the protein. The protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!

References for anti-CHRNB1 antibody (ABIN1742452)

Duttaroy, Gomeza, Gan, Siddiqui, Basile, Harman, Smith, Felder, Levey, Wess: "Evaluation of muscarinic agonist-induced analgesia in muscarinic acetylcholine receptor knockout mice." in: Molecular pharmacology, Vol. 62, Issue 5, pp. 1084-93, (2002) (PubMed).

Bernard, Laribi, Levey, Bloch: "Subcellular redistribution of m2 muscarinic acetylcholine receptors in striatal interneurons in vivo after acute cholinergic stimulation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 23, pp. 10207-18, (1998) (PubMed).

Levey, Edmunds, Hersch, Wiley, Heilman: "Light and electron microscopic study of m2 muscarinic acetylcholine receptor in the basal forebrain of the rat." in: The Journal of comparative neurology, Vol. 351, Issue 3, pp. 339-56, (1995) (PubMed).

Target Details for CHRNB1

Target: CHRNB1 (Nicotinic Acetylcholine Receptor beta (CHRNB1))

Alternative Name: AChR-2

Background:

Synonyms: m-AChR-2, Acetylcholine Receptor 2, Chrm2, muscarinic acetylcholine receptor, Muscarinic acetylcholine receptor M2, Cholinergic receptor muscarinic 2, spinal cord, striatum

Antigen Information: m-AChR-2 is a G protein-coupled acetylcholine receptor

UniProt: P08172

Pathways: Synaptic Membrane