GABRA1 antibody (N-Term)

Catalog No. ABIN1742457

This anti-GABRA1 antibody is a Guinea Pig Polyclonal antibody detecting GABRA1 in WB and ICC. Suitable for Rat and Mouse.

Quick Overview for GABRA1 antibody (N-Term) (ABIN1742457)

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Reactivity: Rat, Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This GABRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-GABRA1 Antibody

Binding Specificity: N-Term

Purpose: anti-GABA-A receptor alpha1

Specificity: Specific for GABA-A receptor alpha1.

Cross-Reactivity (Details): Reacts with: rat (P62813), mouse (P62812). Other species not tested yet.

Purification: affinity purified

Immunogen: Synthetic peptide/recombinant protein corresponding to residues near the amino terminus from rat GABA-A receptor alpha1. (UniProt Id: P62813)

Application Details

Application Notes: WB:1 : 1000 up to 1 : 2000 , IHC-P:not tested yet

Comment:

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

Target Details for GABRA1

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Alternative Name: GABA-A receptor alpha1

Background: GABA receptor, Gabra1

UniProt: P62813