ENO2/NSE antibody (AA 418-434)

Catalog No. ABIN1742474

The Rabbit Polyclonal anti-ENO2/NSE antibody has been validated for WB, IHC and IP. It is suitable to detect ENO2/NSE in samples from Mouse and Rat.

Quick Overview for ENO2/NSE antibody (AA 418-434) (ABIN1742474)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-ENO2/NSE Antibody

Binding Specificity: AA 418-434

Specificity: Specific for gamma-enolase.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide (aa 418-434 of rat gamma-enolase) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: not tested yet
IHC: 1 : 1000 up to 1 : 5000
IHC-P: 1 : 1000 up to 1 : 5000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: gamma-Enolase

Background: Synonyms: Enolase 2, NSE, Neural Enolase