VAMP7 antibody (AA 119-188)

Catalog No. ABIN1742477

The Mouse Monoclonal anti-VAMP7 antibody has been validated for WB and IHC. It is suitable to detect VAMP7 in samples from Mouse and Rat. There are 3+ publications available.

Quick Overview for VAMP7 antibody (AA 119-188) (ABIN1742477)

Target: VAMP7 (Vesicle-Associated Membrane Protein 7 (VAMP7))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This VAMP7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 158-2

Product Details anti-VAMP7 Antibody

Binding Specificity: AA 119-188

Specificity: Specific for VAMP 7.

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Recombinant mouse VAMP 7 (aa 119-188).

Isotype: IgG2a

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 up to 1 : 200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-VAMP7 antibody (ABIN1742477)

Akiyama, Fukuda, Tojima, Nikolaev, Kamiguchi: "Cyclic Nucleotide Control of Microtubule Dynamics for Axon Guidance." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 20, pp. 5636-49, (2016) (PubMed).

Koseoglu, Peters, Fitch-Tewfik, Aisiku, Danglot, Galli, Flaumenhaft: "VAMP-7 links granule exocytosis to actin reorganization during platelet activation." in: Blood, Vol. 126, Issue 5, pp. 651-60, (2015) (PubMed).

Muzerelle, Alberts, Martinez-Arca, Jeannequin, Lafaye, Mazié, Galli, Gaspar: "Tetanus neurotoxin-insensitive vesicle-associated membrane protein localizes to a presynaptic membrane compartment in selected terminal subsets of the rat brain." in: Neuroscience, Vol. 122, Issue 1, pp. 59-75, (2003) (PubMed).

Target Details for VAMP7

Target: VAMP7 (Vesicle-Associated Membrane Protein 7 (VAMP7))

Alternative Name: VAMP 7

Background: Synonyms: Ti-Vamp, SybL 1