Caspase 3 antibody (AA 170-175)

Catalog No. ABIN1742483

The Rabbit Polyclonal anti-Caspase 3 antibody has been validated for ICC. It is suitable to detect Caspase 3 in samples from Human, Mouse, Rat and Cow.

Quick Overview for Caspase 3 antibody (AA 170-175) (ABIN1742483)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Immunocytochemistry (ICC)

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 170-175

Specificity: Recognizes cleaved and activated caspase 3.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide (aa 170-175 of mouse caspase 3) coupled to KLH.

Application Details

Application Notes: WB: not tested yet
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase 3

Background: Synonyms: CPP-32, Apopain, Yama, SCA-1

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation