CLIP1 antibody (AA 1283-1438)

Catalog No. ABIN1742487

This anti-CLIP1 antibody is a Guinea Pig Polyclonal antibody detecting CLIP1 in WB and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for CLIP1 antibody (AA 1283-1438) (ABIN1742487)

Target: CLIP1 (CAP-GLY Domain Containing Linker Protein 1 (CLIP1))

Reactivity: Human, Mouse, Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This CLIP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-CLIP1 Antibody

Binding Specificity: AA 1283-1438

Specificity: Recognizes CLIP 170 and restin.

Purification: Affinity purified with the immunogen. Guinea pig serum albumin was added for stabilization.

Immunogen: Recombinant protein of human CLIP 170 (aa 1283-1438).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: not tested yet

Comment:

ICC: Fix 15 min at -20 °C with ice-cold MeOH / 1mM EGTA, then transfer without washing to 4% PFA (in PBS) and fix for 20 min at RT.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Target Details for CLIP1

Target: CLIP1 (CAP-GLY Domain Containing Linker Protein 1 (CLIP1))

Alternative Name: CLIP 170

Background: Synonyms: Restin

Pathways: Microtubule Dynamics