Glu-alpha-Tubulin (C-Term), (glutarylated) antibody

Catalog No. ABIN1742545

This Mouse Monoclonal antibody specifically detects Glu-alpha-Tubulin in IHC, WB, ICC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Drosophila melanogaster, Eukaryotes, Vertebrate and Zebrafish (Danio rerio). It has been mentioned in 9+ publications

Quick Overview for Glu-alpha-Tubulin (C-Term), (glutarylated) antibody (ABIN1742545)

Target: Glu-alpha-Tubulin

Reactivity: Human, Mouse, Rat, Drosophila melanogaster, Eukaryotes, Vertebrate, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1D5

Product Details

Binding Specificity: C-Term, glutarylated

Purpose: Mouse anti-Glu-Tubulin IgG

Specificity: Specific for detyrosinated alpha-tubulin (glu-tubulin) and polyglutamylated tubulin (also beta-tubulin).

Cross-Reactivity (Details): Reacts with: human (Q71U36), rat (P68370), mouse (P68369), zebrafish, eukaryotes, other vertebrates, Drosophila melanogaster. Other species not tested yet.

Purification: purified IgG

Immunogen: Synthetic peptide corresponding to residues near the c-terminus of human Glu-alpha-tubulin. (UniProt Id: Q71U36) Epitop AA 448 to 450 from human Glu-alpha-tubulin (UniProt Id: Q71U36)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 1000 , IP:not tested yet ICC:1 : 200 up to 1 : 500, IHC:1 : 500, IHC-P:1 : 1000,

Comment:

WB: Tween 20 (other detergents not yet tested) should not be added to the blocking and washing solution. It greatly diminishes the intensity of the signal.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References

Zink, Grosse, Freikamp, Bänfer, Müksch, Jacob: "Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells." in: Journal of cell science, Vol. 125, Issue Pt 24, pp. 5998-6008, (2013) (PubMed).

Jablonka, Dombert, Asan, Sendtner: "Mechanisms for axon maintenance and plasticity in motoneurons: alterations in motoneuron disease." in: Journal of anatomy, Vol. 224, Issue 1, pp. 3-14, (2013) (PubMed).

Roll-Mecak, Vale: "Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin." in: Nature, Vol. 451, Issue 7176, pp. 363-7, (2008) (PubMed).

Zhang, Gilquin, Khochbin, Matthias: "Two catalytic domains are required for protein deacetylation." in: The Journal of biological chemistry, Vol. 281, Issue 5, pp. 2401-4, (2006) (PubMed).

Rosales-Nieves, Johndrow, Keller, Magie, Pinto-Santini, Parkhurst: "Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire and Cappuccino." in: Nature cell biology, Vol. 8, Issue 4, pp. 367-76, (2006) (PubMed).

Yoon, Shin, Mercurio: "Hypoxia stimulates carcinoma invasion by stabilizing microtubules and promoting the Rab11 trafficking of the alpha6beta4 integrin." in: Cancer research, Vol. 65, Issue 7, pp. 2761-9, (2005) (PubMed).

Van der Heiden, Groenendijk, Hierck, Hogers, Koerten, Mommaas, Gittenberger-de Groot, Poelmann: "Monocilia on chicken embryonic endocardium in low shear stress areas." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 235, Issue 1, pp. 19-28, (2005) (PubMed).

Rüdiger, Rüdiger, Wehland, Weber: "Monoclonal antibody ID5: epitope characterization and minimal requirements for the recognition of polyglutamylated alpha- and beta-tubulin." in: European journal of cell biology, Vol. 78, Issue 1, pp. 15-20, (1999) (PubMed).

Wheatley, Feilen, Yin, Wheatley: "Primary cilia in cultured mammalian cells: detection with an antibody against detyrosinated alpha-tubulin (ID5) and by electron microscopy." in: Journal of submicroscopic cytology and pathology, Vol. 26, Issue 1, pp. 91-102, (1994) (PubMed).

Target Details

Target: Glu-alpha-Tubulin

Background:

Synonyms: alpha-tubulin, TUBA1, Glu-tubulin, polyglu-tubulin, polyglutamylated tubulin, polyglu alpha-tubulin, polyglutamylated alpha-tubulin

Antigen Information: alpha-Tubulin is a major cytoskeleton protein

UniProt: Q71U36