TUBB3 antibody (C-Term)

Catalog No. ABIN1742554

This Guinea Pig Polyclonal antibody specifically detects TUBB3 in WB, IHC, IP, ICC and IHC (p). It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for TUBB3 antibody (C-Term) (ABIN1742554)

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivity: Human, Mouse, Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TUBB3 Antibody

Binding Specificity: C-Term

Purpose: anti-beta3-Tubulin antiserum

Specificity: Specific for beta3-tubulin.

Cross-Reactivity (Details): Reacts with: human (Q13509), rat (Q4QRB4), mouse (Q9ERD7). Other species not tested yet.

Purification: antiserum

Immunogen: Synthetic peptide corresponding to residues near the carboxy terminus of mouse beta3-tubulin (UniProt Id: Q9ERD7)

Application Details

Application Notes: WB:1 : 1000 up to 1 : 10000 , IP:yes ICC:1 : 500, IHC:1 : 200, IHC-P:1 : 200 up to 1 : 500,

Comment:

IHC: For best results we recommend antigen retrieval with pepsin according to Lorincz A & Nusser Z (2008).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O, then aliquot and store at -20 °C until use.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

References for anti-TUBB3 antibody (ABIN1742554)

Matthäus, Haddjeri, Sánchez, Martí, Bahri, Rovera, Schloss, Lau: "The allosteric citalopram binding site differentially interferes with neuronal firing rate and SERT trafficking in serotonergic neurons." in: European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology, Vol. 26, Issue 11, pp. 1806-1817, (2016) (PubMed).

Andres, Keyser, Petrali, Benton, Hubbard, McNutt, Ray: "Morphological and functional differentiation in BE(2)-M17 human neuroblastoma cells by treatment with Trans-retinoic acid." in: BMC neuroscience, Vol. 14, pp. 49, (2013) (PubMed).

Lau, Heimann, Bartsch, Schloss, Weber: "Nongenomic, glucocorticoid receptor-mediated regulation of serotonin transporter cell surface expression in embryonic stem cell derived serotonergic neurons." in: Neuroscience letters, Vol. 554, pp. 115-20, (2013) (PubMed).

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: beta3-Tubulin

Background:

Synonyms: TuJ1, TUBB3, Class III ß-Tubulin, ßIII-Tubulin, betaIII-Tubulin, eye-retina

Antigen Information: beta3-Tubulin also known as TuJ1 is a neuron specific Tubulin

UniProt: Q9ERD7

Pathways: Microtubule Dynamics, M Phase