Profilin antibody (AA 1-140)

Catalog No. ABIN1742559

This anti-Profilin antibody is a Mouse Monoclonal antibody detecting Profilin in WB, IHC and IHC (p). Suitable for Human, Cow, Guinea Pig, Opossum and Rabbit. This Primary Antibody has been cited in 6+ publications.

Quick Overview for Profilin antibody (AA 1-140) (ABIN1742559)

Target: Profilin (PFN)

Reactivity: Human, Cow, Guinea Pig, Opossum, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Profilin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2H11

Product Details anti-Profilin Antibody

Binding Specificity: AA 1-140

Purpose: Mouse anti-Bovine Profilin IgG

Specificity: Specific for profilin 1.

Cross-Reactivity (Details): Reacts with: human (P07737), cow, rabbit, Guinea pig, opossum. No signal: mouse (P62962). Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 1 to 140 from bovine Profilin1 (UniProt Id: P02584)

Isotype: IgG1

Application Details

Application Notes: WB:1 : 500 up to 1 : 5000 IHC:yes IHC-P:1 : 1000,

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!

References for anti-Profilin antibody (ABIN1742559)

Lorente, Syriani, Morales: "Actin filaments at the leading edge of cancer cells are characterized by a high mobile fraction and turnover regulation by profilin I." in: PLoS ONE, Vol. 9, Issue 1, pp. e85817, (2014) (PubMed).

Giesemann, Schwarz, Nawrotzki, Berhörster, Rothkegel, Schlüter, Schrader, Schindelin, Mendel, Kirsch, Jockusch: "Complex formation between the postsynaptic scaffolding protein gephyrin, profilin, and Mena: a possible link to the microfilament system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 23, pp. 8330-9, (2003) (PubMed).

Giesemann, Rathke-Hartlieb, Rothkegel, Bartsch, Buchmeier, Jockusch, Jockusch: "A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems." in: The Journal of biological chemistry, Vol. 274, Issue 53, pp. 37908-14, (2000) (PubMed).

Janke, Schlüter, Jandrig, Theile, Kölble, Arnold, Grinstein, Schwartz, Estevéz-Schwarz, Schlag, Jockusch, Scherneck: "Suppression of tumorigenicity in breast cancer cells by the microfilament protein profilin 1." in: The Journal of experimental medicine, Vol. 191, Issue 10, pp. 1675-86, (2000) (PubMed).

Schlüter, Schleicher, Jockusch: "Effects of single amino acid substitutions in the actin-binding site on the biological activity of bovine profilin I." in: Journal of cell science, Vol. 111 ( Pt 22), pp. 3261-73, (1999) (PubMed).

Wiedemann, Giehl, Almo, Fedorov, Girvin, Steinberger, Rüdiger, Ortner, Sippl, Dolecek, Kraft, Jockusch, Valenta: "Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody." in: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29915-21, (1997) (PubMed).

Target Details for Profilin

Target: Profilin (PFN)

Alternative Name: Profilin

Background:

Synonyms: profilin

Antigen Information: Profilins are regulators of the microfilament network

UniProt: P02584