Profilin antibody

Catalog No. ABIN1742559

Product Details anti-Profilin Antibody

Target: Profilin (PFN)

Reactivity: Human, Cow, Guinea Pig, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Profilin antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Specificity: Specific for profilin 1.

Purification: purified IgG

Immunogen: Fusion protein of alpha-actinin from dictyostelium and bovine profilin.

Clone: 2H11

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 500 up to 1 : 5000 (AP staining)
IP: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Profilin antibody (ABIN1742559)

Lorente, Syriani, Morales: "Actin filaments at the leading edge of cancer cells are characterized by a high mobile fraction and turnover regulation by profilin I." in: PLoS ONE, Vol. 9, Issue 1, pp. e85817, (2014) (PubMed).

Giesemann, Schwarz, Nawrotzki, Berhörster, Rothkegel, Schlüter, Schrader, Schindelin, Mendel, Kirsch, Jockusch: "Complex formation between the postsynaptic scaffolding protein gephyrin, profilin, and Mena: a possible link to the microfilament system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 23, pp. 8330-9, (2003) (PubMed).

Giesemann, Rathke-Hartlieb, Rothkegel, Bartsch, Buchmeier, Jockusch, Jockusch: "A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems." in: The Journal of biological chemistry, Vol. 274, Issue 53, pp. 37908-14, (2000) (PubMed).

Janke, Schlüter, Jandrig, Theile, Kölble, Arnold, Grinstein, Schwartz, Estevéz-Schwarz, Schlag, Jockusch, Scherneck: "Suppression of tumorigenicity in breast cancer cells by the microfilament protein profilin 1." in: The Journal of experimental medicine, Vol. 191, Issue 10, pp. 1675-86, (2000) (PubMed).

Schlüter, Schleicher, Jockusch: "Effects of single amino acid substitutions in the actin-binding site on the biological activity of bovine profilin I." in: Journal of cell science, Vol. 111 ( Pt 22), pp. 3261-73, (1999) (PubMed).

Wiedemann, Giehl, Almo, Fedorov, Girvin, Steinberger, Rüdiger, Ortner, Sippl, Dolecek, Kraft, Jockusch, Valenta: "Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody." in: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29915-21, (1997) (PubMed).

Target Details for Profilin

Target: Profilin (PFN)

Alternative Name: Profilin (PFN Products)

Synonyms: LOC406154 antibody, GB13380 antibody, profilin antibody, PFN antibody, PRF antibody