tau antibody (N-Term)

Catalog No. ABIN1742567

The Mouse Monoclonal anti-tau antibody has been validated for WB, IHC, IHC (p), ICC and IP. It is suitable to detect tau in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for tau antibody (N-Term) (ABIN1742567)

Target: tau (tau Protein)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This tau antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 248E5

Product Details anti-tau Antibody

Binding Specificity: N-Term

Purpose: Mouse anti-Tau IgG

Specificity: Specific for tau.

Cross-Reactivity (Details): Reacts with: rat (P19332), mouse (P10637). Weaker signal: human (P10636). No signal: zebrafish. Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to the N-terminal half of mouse Tau-D (UniProt Id: P10637-5)

Isotype: IgG2a

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:1 : 200 up to 1 : 500, IHC:1 : 100, IHC-P:1 : 500,

Comment:

WB: Detects the mouse protein with much greater sensitivity than the rat protein. The antibody binds phosphorylated and non-phosphorylated tau proteins.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-tau antibody (ABIN1742567)

Gut, Beske, Hubbard, Lyman, Hamilton, McNutt: "Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury." in: PLoS ONE, Vol. 8, Issue 5, pp. e64423, (2013) (PubMed).

Ahmad, Wolber, Eckardt, Koch, Schmitt, Semechkin, Geis, Heckmann, Brüstle, McLaughlin, Sirén, Müller: "Functional neuronal cells generated by human parthenogenetic stem cells." in: PLoS ONE, Vol. 7, Issue 8, pp. e42800, (2012) (PubMed).

Target Details for tau

Target: tau (tau Protein)

Alternative Name: Tau

Background:

Synonyms: Microtubule-associated protein tau, Mapt, MAPT

Antigen Information: Tau is a neuronal microtubule associated protein

UniProt: P10637