CD45 antibody (APC)

Catalog No. ABIN1774737

Product Details anti-CD45 Antibody (APC)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Human, Mouse, Cat

Host: Rat

Clonality: Monoclonal

Conjugate: This CD45 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.

Cross-Reactivity (Details): Human, Mouse, Feline (Cat)

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Abelson murine leukemia virus-induced pre-B tumor cells

Clone: RA3-6B2

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD45 antibody (APC) (ABIN1774737)

Sahai, Jones, Hughes, Pu, Williams, Iyer, Rathinam, Davis, Lovering, Gilotra: "Fibroadipogenic progenitor cell response peaks prior to progressive fatty infiltration after rotator cuff tendon tear." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, (2022) (PubMed).

Montecino-Rodriguez, Leathers, Dorshkind: "Identification of a B-1 B cell-specified progenitor." in: Nature immunology, Vol. 7, Issue 3, pp. 293-301, (2006) (PubMed).

Rothaeusler, Baumgarth: "Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 69, Issue 4, pp. 249-59, (2006) (PubMed).

Cappione, Pugh-Bernard, Anolik, Sanz: "Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4298-307, (2004) (PubMed).

Tsurui, Nishimura, Hattori, Hirose, Okumura, Shirai: "Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 48, Issue 5, pp. 653-62, (2000) (PubMed).

Rada, Ehrenstein, Neuberger, Milstein: "Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting." in: Immunity, Vol. 9, Issue 1, pp. 135-41, (1998) (PubMed).

Watanabe, Akaike: "Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells." in: Scandinavian journal of immunology, Vol. 39, Issue 5, pp. 419-25, (1994) (PubMed).

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45R (PTPRC Products)

Background: CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.,PTPRCR, T200R

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling