Thrombospondin 1 antibody (N-Term)

Catalog No. ABIN1781811

This anti-Thrombospondin 1 antibody is a Goat Polyclonal antibody detecting Thrombospondin 1 in WB, IHC, ELISA, IF and FACS. Suitable for Human and Mouse.

Quick Overview for Thrombospondin 1 antibody (N-Term) (ABIN1781811)

Target: Thrombospondin 1 (THBS1)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Thrombospondin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Thrombospondin 1 Antibody

Binding Specificity: N-Term

Purpose: Thrombospondin

Sequence: NRIPESGGDN SVFD

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 150 kDa band observed in Mouse Kidney lysates (calculated MW of 129 kDa according to NP_035710.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Alternative Name: THBS1

Background: THBS1, thrombospondin 1, THBS, TSP, TSP1, thrombospondin-1p180

Gene ID: 7057

NCBI Accession: NP_003237

Pathways: Autophagy