PRTN3 antibody (AA 88-98)

Catalog No. ABIN1781866

This Goat Polyclonal antibody specifically detects PRTN3 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for PRTN3 antibody (AA 88-98) (ABIN1781866)

Target: PRTN3 (Proteinase 3 (PRTN3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRTN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PRTN3 Antibody

Binding Specificity: AA 88-98

Purpose: proteinase 3 / myeloblastin (aa88-98)

Sequence: HNVRTQEPTQ Q

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 28 kDa band observed in Human Spleen lysates (calculated MW of 27.8 kDa according to NP_002768.3). Recommended concentration:0.3-1 μg/mL. Primary incubation was 1 hour

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PRTN3

Target: PRTN3 (Proteinase 3 (PRTN3))

Alternative Name: PRTN3

Background: PRTN3, proteinase 3, ACPA, AGP7, C-ANCA, CANCA, MBN, MBT, NP4, P29, PR-3, PR3, C-ANCA antigen, NP-4, Wegener granulomatosis autoantigen, azurophil granule protein 7, leukocyte proteinase 3, myeloblastin, neutrophil proteinase 4, serine proteinase, neutrop

Molecular Weight: Expected molecular weight: 27 kDa

Gene ID: 5657

NCBI Accession: NP_002768