ADAM12 antibody (AA 225-239)

Catalog No. ABIN1781942

The Goat Polyclonal anti-ADAM12 antibody has been validated for ELISA and WB. It is suitable to detect ADAM12 in samples from Human, Mouse, Rat and Pig.

Quick Overview for ADAM12 antibody (AA 225-239) (ABIN1781942)

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ADAM12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Verified

Product Details anti-ADAM12 Antibody

Binding Specificity: AA 225-239

Purpose: ADAM12 (aa225-239)

Sequence: REFQRQGKDL EKVKQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_003465.3, NP_067673.2).

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in Human Heart lysates and approx 90+60 kDa bands observed in Mouse, Rat and Pig Heart lysates (calculated MW of 99.5 kDa according to NP_003465.3 and of 80.4 kDa according to NP_067673.2). Recommended concentration

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ADAM12

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Alternative Name: ADAM12

Background: ADAM12, ADAM metallopeptidase domain 12, MCMP, MCMPMltna, MLTN, MLTNA, disintegrin and metalloproteinase domain-containing protein 12, meltrin-alpha

Molecular Weight: Expected molecular weight: 90+60 kDa

Gene ID: 8038, 11489, 679837

NCBI Accession: NP_003465, NP_067673

Pathways: EGFR Signaling Pathway