Cytokeratin 18 antibody (Internal Region)

Catalog No. ABIN1781972

The Goat Polyclonal anti-Cytokeratin 18 antibody has been validated for WB, IHC and ELISA. It is suitable to detect Cytokeratin 18 in samples from Human and Pig.

Quick Overview for Cytokeratin 18 antibody (Internal Region) (ABIN1781972)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Cytokeratin 18 Antibody

Binding Specificity: Internal Region

Purpose: keratin 18

Sequence: EELDKYWSQQ IE

Specificity: Reported variants represent identical protein: NP_000215.1, NP_954657.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Breast, Placenta and Small Intestine. Recommended concentration: 5 μg/mL.

Western Blot: Approx 48 kDa band observed in lysates of cell lines A431, HeLa, MCF7 (calculated MW of 48.1 kDa according to NP_000215.1) and approx 45 kDa in lysates of Pig Liver (calculated MW of 47.4 kDa according to XP_005652636.2). Recommended concentrat

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: KRT18

Background: KRT18, keratin 18, CYK18, K18, CK-18, cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, cytokeratin 18, cytokeratin-18, keratin, type I cytoskeletal 18, keratin-18

Molecular Weight: Expected molecular weight: 48 kDa

Gene ID: 3875, 16668, 294853

NCBI Accession: NP_000215

Pathways: Apoptosis, Caspase Cascade in Apoptosis