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Cytokeratin 18 antibody (Internal Region)

Verified KRT18 Reactivity: Human, Pig WB, IHC, ELISA Host: Goat Polyclonal unconjugated
Catalog No. ABIN1781972
  • Target See all Cytokeratin 18 (KRT18) Antibodies
    Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
    Binding Specificity
    • 25
    • 16
    • 15
    • 14
    • 13
    • 12
    • 7
    • 6
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 240
    • 129
    • 95
    • 41
    • 34
    • 27
    • 22
    • 19
    • 19
    • 18
    • 16
    • 14
    • 14
    • 14
    • 8
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 1
    Human, Pig
    Host
    • 148
    • 125
    • 3
    • 1
    • 1
    Goat
    Clonality
    • 143
    • 134
    • 1
    Polyclonal
    Conjugate
    • 146
    • 23
    • 22
    • 10
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Cytokeratin 18 antibody is un-conjugated
    Application
    • 220
    • 102
    • 86
    • 76
    • 71
    • 56
    • 54
    • 40
    • 38
    • 27
    • 27
    • 19
    • 8
    • 8
    • 7
    • 6
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Purpose
    keratin 18
    Sequence
    EELDKYWSQQ IE
    Specificity
    Reported variants represent identical protein: NP_000215.1, NP_954657.1.
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Isotype
    IgG
    Top Product
    Discover our top product KRT18 Primary Antibody
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Breast, Placenta and Small Intestine. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 48 kDa band observed in lysates of cell lines A431, HeLa, MCF7 (calculated MW of 48.1 kDa according to NP_000215.1) and approx 45 kDa in lysates of Pig Liver (calculated MW of 47.4 kDa according to XP_005652636.2). Recommended concentrat

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
    Alternative Name
    KRT18 (KRT18 Products)
    Background
    KRT18, keratin 18, CYK18, K18, CK-18, cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, cytokeratin 18, cytokeratin-18, keratin, type I cytoskeletal 18, keratin-18
    Molecular Weight
    Expected molecular weight: 48 kDa
    Gene ID
    3875, 16668, 294853
    NCBI Accession
    NP_000215
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
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