Cytokeratin 19 antibody (AA 285-298)

Catalog No. ABIN1781975

The Goat Polyclonal anti-Cytokeratin 19 antibody has been validated for WB, IHC and ELISA. It is suitable to detect Cytokeratin 19 in samples from Human.

Quick Overview for Cytokeratin 19 antibody (AA 285-298) (ABIN1781975)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Cytokeratin 19 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Cytokeratin 19 Antibody

Binding Specificity: AA 285-298

Purpose: cytokeratin 19 (aa285-298)

Sequence: HTEQLQMSRS EVTD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 43 kDa band observed in Human cancer lysates of Breast, Pancreas and Colon (calculated MW of 44.1 kDa according to NP_002267.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: KRT19

Background: KRT19, keratin 19, CK19, K19, K1CS, 40- kDa keratin intermediate filament, CK-19, cytokeratin 19, cytokeratin-19, keratin, type I cytoskeletal 19, keratin, type I, 40-kd, keratin-19

Molecular Weight: Expected molecular weight: 43 kDa

Gene ID: 3880

NCBI Accession: NP_002267