HSF1 antibody (N-Term)

Catalog No. ABIN180344

The Rat Monoclonal anti-HSF1 antibody has been validated for WB, IP and IF. It is suitable to detect HSF1 in samples from Human, Mouse and Rat.

Quick Overview for HSF1 antibody (N-Term) (ABIN180344)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human, Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 4B4

Product Details anti-HSF1 Antibody

Binding Specificity: AA 1-503, N-Term

Specificity: Highly specific for HSF1. Detects an ~85 kDa protein in unstressed cell lysates, and an ~95 kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat, Bovine, Guinea Pig, Hamster, Monkey, RabbitSpecies reactivity (tested):Mouse

Characteristics: Synonyms: HSTF1, HSF-1, Heat shock transcription factor 1

Purification: Immunoglobulin fraction purified by ammonium sulphate precipitation

Immunogen: Recombinant mouse HSF1 protein (aa1-503).

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence. Immunoprecipitation (Native only): use Protein G and Ab at 2g/mgprotein lysate). Western Blotting: Ab 1-2g/mL for 2hrs at RT. Recommended positivecontrol: LS174T or MAD109 Cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS pH 7.4, 50 % Glycerol, 0.09 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one month or in aliquots at -20 °C for longer. Avoid repeated freezethaw cycles!
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: Heat Shock Factor 1 / HSF1

Background: HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents, binds to heat shock response elements (HSEs) in the upstream region of heat shock gene and activates their transcription. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA.Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1

Gene ID: 3297

UniProt: Q00613