SMURF1 antibody (AA 66-96)

Catalog No. ABIN1804041

The Rabbit Polyclonal anti-SMURF1 antibody (ABIN1804041) specifically detects SMURF1 in WB, IHC and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for SMURF1 antibody (AA 66-96) (ABIN1804041)

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMURF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SMURF1 Antibody

Binding Specificity: AA 66-96

Specificity: This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1.

Purification: Ammonium sulfate precipitation

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not been evaluated for use in IHC with mouse samples.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Expiry Date: 6 months

Target Details for SMURF1

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Alternative Name: SMURF1

Background: Name/Gene ID: SMURF1

Synonyms: SMURF1, E3 ubiquitin ligase SMURF1, KIAA1625, HSMURF1

Gene ID: 57154

UniProt: Q9HCE7

Pathways: Regulation of Cell Size