UBC antibody (AA 36-66)

Catalog No. ABIN1804046

The Rabbit Polyclonal anti-UBC antibody has been validated for WB, IHC, IF and IHC (p). It is suitable to detect UBC in samples from Human.

Quick Overview for UBC antibody (AA 36-66) (ABIN1804046)

Target: UBC (Ubiquitin C (UBC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBC Antibody

Binding Specificity: AA 36-66

Brand: IHC-plus™

Specificity: This Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-66 amino acids of human Ubiquitin.

Purification: Ammonium sulfate precipitation

Application Details

Application Notes: Approved: IF (1:10 - 1:50), IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Expiry Date: 6 months

Target Details for UBC

Target: UBC (Ubiquitin C (UBC))

Alternative Name: UBC / Ubiquitin C

Background: Name/Gene ID: UBC

Synonyms: UBC, HUBCEP52, L40, HMG20, RPL40, Ubiquitin, Polyubiquitin-C, CEP52, Ubiquitin C

Gene ID: 7316

UniProt: P0CG48

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, EGFR Downregulation, Ubiquitin Proteasome Pathway